Anti-SPPase1抗体 YB--17659R报价/价格-抗体 Anti-SPPase1抗体 Y

生物论坛 抗体

 Anti-SPPase1抗体

产品编号 YB-17659R 英文名称 SPPase1 中文名称 ***酸鞘氨醇***酸酶抗体 别    名 Sphingosine 1 phosphate phosphatase 2; hSPPase1; SGPP1; Sphingosine-1-phosphatase 1; Spp1; SPPase1.   规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价 说 明 书 100ul  200ul 研究领域 细胞生物  免疫学  神经生物学  激酶和***酸酶   抗体来源 Rabbit 克隆类型 Polyclonal 交叉反应 Human, Mouse, Rat, Dog, Cow, Horse,  产品应用 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分 子 量 45kDa 细胞定位 细胞浆 细胞膜  性    状 Lyophilized or Liquid 浓    度 1mg/ml 免 疫 原 KLH conjugated synthetic peptide derived from human SPPase1:1-100/399  亚    型 IgG 纯化方法 affinity purified by Protein A 储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. PubMed PubMed 产品介绍 background: Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides (Mandala et al., 2000 [PubMed 10859351]; Le Stunff et al., 2007 [PubMed 17895250]).[supplied by OMIM, Jun 2009] Function: Sphingosine-1-phosphate phosphatase 1 (Spp1) regulates intracellular and extracellular sphingosine 1 phosphate(S1P)levels. It belongs to the type 2 lipid phosphate phosphatase family and is expressed in the endoplasmic reticulum (ER) membrane. Subunit: Has specific phosphohydrolase activity towards sphingoid base 1-phosphates. Has high phosphohydrolase activity against dihydrosphingosine-1-phosphate and sphingosine-1-phosphate (S1P) in vitro. May play a role in attenuating intracellular sphingosine 1-phosphate (S1P) signaling. May play a role in pro-inflammatory signaling.  Subcellular Location: Endoplasmic reticulum membrane Tissue Specificity: Expressed strongly in kidney and heart, followed by brain, colon, small intestine and lung. Not detected in skeletal muscle, thymus, spleen, liver, placenta, and peripheral blood leukocytes.  Similarity: Belongs to the type 2 lipid phosphate phosphatasefamily.  SWISS: Q9BX95 Gene ID: 81537 Database links:

Entrez Gene: 81537 Human

Entrez Gene: 81536 Rat

SwissProt: Q9BX95 Human

SwissProt: Q9JI99 Mouse

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    产品图片
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPPase1) Polyclonal Antibody, Unconjugated (bs-17659R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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